Maintenance work will be completed this weekend.
Echuca residents planning to travel to Melbourne this weekend are urged to plan ahead.
Coaches will replace Bendigo and Echuca trains between Southern Cross Station and Gisborne from the first service on Saturday 10 August through to last service on Sunday 11 August.
Coaches will connect with trains at Gisborne, then run to Bendigo and Echuca.
This comes as vital track maintenance is underway at the new Melton Highway road bridge at Sydenham.
Important track maintenance works would also be completed at the same time between Sunbury and Sunshine to help minimise disruptions to passengers.
Passengers should allow up to 60 minutes additional travel time.
Pets, hot food and liquids cannot be taken on replacement coaches.